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Tag : Purifying Proteins

Review of Basic Techniques and Purifying Proteins

Written by Kasia Bender Objectives: –       To practice calculating various solution components, including molarity and % (m/v). –       To practice the use of a graduated pipet and micropipette. –       To review lab safety. Part I A: Calculations   Part I B: Table One: Sample Conc. (µg/µL) glucose (µL) buffer (µL) 1 0.0 0.0 187.5 2 1.0 30.0 187.5 3 2.0 60.0 187.5 4 3.0 90.0 187.5 5 4.0 120.0 187.5 6 5.0 150.0 187.5 Table Two: Stock MM vol. MM […]