Tag : Automated Sequencing

Automated Sequencing of the psaB Gene Using LI-COR 4200 DNA Analyzer

Written by Erin Downey INTRODUCTION             A section of cloned DNA can be sequenced by a variety of methods, the most common of which is the Sanger method. The Sanger method uses low concentrations of dideoxynucleoside triphosphates (ddNTPs) to act as chain terminators. These are used with the normal, unmodified deoxynucleoside triphosphates. Terminators are separated into ddATP, ddTTPs, ddCTPs, and ddGTPs samples so that when they are sequenced, each lane identifies the terminal nucleotide. This results in fragments of varying […]