Tag : cholesterol

Separating Benzoic Acid, 4-choloraniline and cholesterol using extraction

Written by Brandon Ritter What does it mean to “work up” an organic reaction? Purpose: The purpose of this lab was to separate benzoic acid, 4-choloraniline and cholesterol using extraction. Reaction Equations: Missing 🙁 Structures: Calculations: Missing 🙁 Results: Benzoic Acid: Melting Point: 121.9-123.1⁰C Mass: 0.369g 4-chloroaniline: Melting Point: 68.6-70.2⁰C Mass: 0.043g Cholesterol: Melting Point: 146.7-147.2⁰C Mass: 0.205g Discussion: Our percent recovery for benzoic acid was really good, we got a lot more recovered benzoic acid product than expected. We got slightly […]

Bromination of Cholesterol and Debromination: Purification of Cholesterol

Written by: Nicholas Connor The purpose of this experiment was to understand alkene-halide reactions by purifying commercial cholesterol. Analysis The purpose of this experiment was to remove the impurities present in commercial cholesterol; to yield pure 5-Cholesten-3B-ol. Bromination was the method chosen to remove traces of 3B-cholestanol, 7-cholesten-3b-ol, and 5,7-cholestadien-3B-ol that were assumed to be present in the sample used. The net reaction of bromination follows: To begin removing the impurities, the cholesterol sample (0.97 g) was dissolved in tert-butyl-methyl-ether […]