Tag : mutagenesis

pGLO and Mutagenesis

Written by: Nathan Sheppard Abstract             Plasmids are pieces of circular DNA that are in bacteria which can code for genes that can cause the bacteria to have extra “features”. With the pGLO plasmid this extra “feature” causes bacteria to glow (from the Green Fluorescent Protein that was inserted) and also has resistance to ampicillin (from the beta-lactamase protein). By adding the pGLO plasmid the E.coli bacteria will be able to grow on agar with arabinose sugar and ampicillin. The […]