Spectroscopy & Protein Characterization: SDS PAGE and Spectroscopic Analysis of an Unknown Protein

Written by Emily ABSTRACT: Characterizing proteins is useful for determining structural, functional, and other biologically important information. In this experiment, an unknown protein (E) was analyzed, and was found to weigh approximately 50kDa (50,000 g/mol) and have a concentration of 1.56 mg/mL. This data was found using absorbance spectroscopy and SDS-PAGE. These are just two techniques for analyzing proteins out of a plethora of techniques available, and the data gathered from these techniques is useful, but not enough to form […]