Category : Natural Sciences

Bromination of Cholesterol and Debromination: Purification of Cholesterol

Written by: Nicholas Connor The purpose of this experiment was to understand alkene-halide reactions by purifying commercial cholesterol. Analysis The purpose of this experiment was to remove the impurities present in commercial cholesterol; to yield pure 5-Cholesten-3B-ol. Bromination was the method chosen to remove traces of 3B-cholestanol, 7-cholesten-3b-ol, and 5,7-cholestadien-3B-ol that were assumed to be present in the sample used. The net reaction of bromination follows: To begin removing the impurities, the cholesterol sample (0.97 g) was dissolved in tert-butyl-methyl-ether […]

Lab Report: E. Coli transformation with GFP

E.Coli by Definition: a bacterium commonly found in the intestines of humans and other animals, where it usually causes no harm. Some strains can cause severe food poisoning, especially in old people and children. What organism does this lab attempt to transform? This lab attempts to transform bacterial E. Coli. In the context of this lab, what do we mean when we say a cell is competent? Cellular competence is the ability of a cell to take up genetic material […]

Lab Parts: Evaluating Spring Constants with the Least Squares Method of Data Fitting

Student Researched Lab Experiment to evaluate Spring Constants Introduction In this experiment we evaluated Hooke’s law by measuring the stretch of a spring with incremental masses. A motion detector provided position measurements and the masses were added 100 grams at a time. To calculate the spring constant we utilized the least squares method of data fitting on a Force by distance graph. Samples Calculations Line of Best Fit: f(x) = 0.202x + 16.5 xi-mean height, yi-force of gravity (1) Mean […]

Lab Parts: Ball Toss & Fluid Resistance

Introduction In this experiment, a motion detector was used to collect distance, velocity, and acceleration data for different types of balls being thrown straight up in the air. Like a juggler who tosses a ball straight upward, each ball slows down on it’s path to maximum height, then begins to speed up on it’s way down. The concept is that there is a definite mathematical pattern to the changes in velocity, which will be reinforced by experimentation. Analysis (2) How […]

Alternative Energy: Solar Power

Written by: Nicholas Connor In spite of their evident disadvantages, fossil fuels have been the dominant energy source of the 20th century. Perhaps as Brian Cox has asserted in Chain Reaction, “we are addicted to petroleum”. Environmental consequences thereof, such as carbon dioxide emissions and recent industrial spills, implicate that the contiguous manufacture and consumption of these fuels are perilous for future generations. Although society celebrates consumer technology, Max Planck’s conjecture is pertinent: “A new scientific truth does not triumph […]