Tag : Beer’s Law

BCA Protein Concentration Assay

Objective Utilizing Beer’s law, known concentrations of bovine serum albumin (BSA) are reacted with bicinchonic acid (BCA) to create a standard curve of absorbance vs. concentration of protein.  This curve is then utilized to determine the concentration of protein in an unknown solution. Introduction Beer’s law () tells us that the light absorbance (A) of a solution is proportional to the concentration of the protein (c) within the solution, the light path (b) and the extinction coefficient of the protein.  […]

Rate Law Determination of a Crystal Violet Reaction

Objective: To determine the reaction order with respect to crystal violet and determine the rate constant and half life for the reaction: [CV+ + OH– Þ CVOH]. Procedure: (1) Calibrate the Colorimeter with distilled water. (2) Prepare a solution with 10ml each of the provided CV and OH. (3) Let solution stand for 3 minutes before beginning (4). (4) Place solution in Colorimeter and record data point with software. (5) Remove solution for one minute. (6) Repeat steps (4-5) twenty […]