Tag : E. Coli

Transfer of Escherichia Coli

Transfer of Escherichia Coli By: Amudha Venugopalan, Sarah Councilor, and David Roehrich Introduction: Escherichia coli are prokaryotic bacteria that can be transformed by lateral gene transfer. E. coli are transformed by direct transfer which means the bacterial cell directly uptakes the DNA of the plasmid introduced to it (Wimpee et al. 2013). A plasmid is a circular small piece of self-replicating DNA, plasmids carry fewer genes therefore DNA can be passed between species of bacteria (Wimpee et al. 2013). The […]

Determination of lac Operon Functionality of Two Strains of E. coli by Comparing β-galactosidase Activity

By Christopher Cronkite: Abstract             Operons are regions of prokaryotic DNA that contain multiple genes without an interfering termination sequence. Because of this, the genes in an operon are all transcribed into a single mRNA. In this way, a cell can regulate transcription of multiple, related genes using the same mechanisms. In Escherichia coli, the lac operon codes for three proteins involved in lactose catabolism. Expression of this operon is regulated by both a repressor and enhancer that function dependent […]

Lab Report: E. Coli transformation with GFP

E.Coli by Definition: a bacterium commonly found in the intestines of humans and other animals, where it usually causes no harm. Some strains can cause severe food poisoning, especially in old people and children. What organism does this lab attempt to transform? This lab attempts to transform bacterial E. Coli. In the context of this lab, what do we mean when we say a cell is competent? Cellular competence is the ability of a cell to take up genetic material […]