Tag : Protein Concentration

BCA Protein Concentration Assay

Objective Utilizing Beer’s law, known concentrations of bovine serum albumin (BSA) are reacted with bicinchonic acid (BCA) to create a standard curve of absorbance vs. concentration of protein.  This curve is then utilized to determine the concentration of protein in an unknown solution. Introduction Beer’s law () tells us that the light absorbance (A) of a solution is proportional to the concentration of the protein (c) within the solution, the light path (b) and the extinction coefficient of the protein.  […]

Measuring Protein Concentration

By: Amanda Campbell Introduction: This experiment was performed in order to explore three different methods of measuring protein concentration: the Bradford method, the UV method, and the Biuret method. All three methods are quick methods to determine the concentration of protein in a solution, although none can determine the concentration of a specific protein in a solution of proteins. The Bradford method uses a blue dye, Coomassie Brilliant Blue G-250, which binds to positively charged side groups on amino acids. […]