Tag : spectroscopy

Comparing Tris-2,2’-bipyridine Complexes of Iron (II) and Ruthenium (II) through Spectroscopy and Electrochemistry

Written by Johanna ABSTRACT: This experiment effectively determined the differences between Iron (II) and Ruthenium (II) Tris-2,2’-bipyridine complexes through the comparison of absorption and emission spectroscopy and cyclic voltammetry.  In both the UV/Vis absorption and emission spectra, the Ru (II) complex shows far greater molar absorptivity values and fluorescence.  The iron complex does not fluoresce.  The MLCT peaks in the UV/Vis spectra correspond to the energy of the t2g à t2g*  or HOMO à LUMO transitions, 2.64 x 105 J/mol […]

Measuring Mass of Yellow Dye and Sparkler’s Cations with Spectroscopy

Written by Hannah Purpose To measure the grams of yellow 5 dye in a sample (i.e. bottle of Minute Maid Lemonade), and to identify the cations present in sparklers using their emission spectra (Spectroscopy).   Procedure Six standard solutions were made from the Yellow 6 stock solution. The first standard solution was made by adding 5.00 mL 6.550×10-5 M yellow 6 solution and 2.00 mL of distilled water to a test tube using a graduated pipet and by subsequently mixing […]

Spectroscopy & Protein Characterization: SDS PAGE and Spectroscopic Analysis of an Unknown Protein

Written by Emily ABSTRACT: Characterizing proteins is useful for determining structural, functional, and other biologically important information. In this experiment, an unknown protein (E) was analyzed, and was found to weigh approximately 50kDa (50,000 g/mol) and have a concentration of 1.56 mg/mL. This data was found using absorbance spectroscopy and SDS-PAGE. These are just two techniques for analyzing proteins out of a plethora of techniques available, and the data gathered from these techniques is useful, but not enough to form […]

Raman Spectroscopy of Benzene

Abstract Introduction: In Raman Spectroscopy is a powerful technique to analyze different compounds and has a variety of applications. The theory behind this is derived from the Raman Effect (1) The Raman effect refers to the scattering of light after it interacts with a molecule. When a molecule interacts with light, there is a change in the intensity of the light between the incoming and outgoing intensities. This can be described via the Beer Lambert law in equation 1. (2) […]