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Tag : gas chromatography

Separation & Identification of Alcohols by Gas Chromatography

Introduction This experiment explored aspects of gas chromatography to quantitatively and qualitatively study mixtures. This technique involves separating mixtures to gain a better understanding of the sample’s composition. Gas chromatography uses an injector port that feeds into one of two columns where the mixture is separated. The injected sample will be vaporized by the high temperature and travel down the column. The column itself comes in two varieties: packed and capillary. Packed columns are tubes that have particles that are […]

Gas Chromatography to Determination of Percent Alcohol in Crest Pro Health and Listerine Mouthwashes

Determination of Percent Alcohol in Crest Pro Health and Listerine Mouthwashes via Gas Chromatography By: Kiley Morgan, JT Ray, Amy Sizemore, Andrew Puetz Introduction             Mouthwash is an antiseptic solution used as a hygienic product for oral health. This antiseptic can kill bacteria, prevent and combat plaque buildup, gingivitis, and bad breathe. The two mouthwashes analyzed in this experiment are Crest Pro Health Mouthwash and Listerine Mouthwashes. These two mouthwashes contain different active ingredients. Listerine’s active ingredients to kill bacteria […]

Gas Chromatography to Identify Orange Oil in a n-Hexane Unknown

Written by Juan Purpose: The purpose of this experiment was to determine the percentage of orange oil in an n-hexane unknown solution by analyzing the wt% of d-limonene through gas chromatography, using anisole as an internal standard. Sample Calculations Weight of Anisole in Standard #1     Weight of Limonene in Standard #1     Limonene: Anisole weight ratio in Standard #1     Limonene: Anisole peak area ratio in Standard #1   LLS Slope, intercept, and equation for internal […]

Sodium Borohydride Reduction of 2-methylcylohexanone

Purpose The object of this experiment was to reduce the ketone group of a cycloalkane into an alcohol group. This reduction forms cis and trans isomers and the experiment intended to determine which was more favorable. Analysis The object of this experiment was to reduce the ketone group of 2-methylcyclohexanone to form 2-methylcyclohexanol (see figure 1 below). Infrared spectroscopy, an important analytical technique, was introduced to examine the purity of the product. Infrared spectroscopy analyzes a chemical’s transmittance over a […]