Tag : spectrophotometry

Determining the pKa of Bromothymol Blue by spectrophotometry

James Robertson Chemistry 2220 Determining the pKa of Bromothymol Blue by spectrophotometry Abstract To determine the pKa of an acid-base indicator, specifically bromothymol blue, different amounts of both 0.1M NaOH and 0.1 M HCl were added to a solution of fixed concentration of bromothymol blue and the pH was monitored via pH meter. At different pH readings around the researched pKa of Bromothymol blue we measured absorbance of the buffer solution. By superimposing the absorbance graphs, two wavelengths of absorbance […]

Fluorescence Spectrophotometry of Riboflavin

Fluorescence Spectrophotometry of Riboflavin By: Andrew Tam Introduction This experiment was done to explore an aspect of spectroscopic analysis called fluorescence. This aspect involves shining an ultraviolet light, which will excite the electrons of certain molecules. When these excited electrons returned to their ground (original) state, energy is released and causes the molecule to emit light. This light can then be captured with the use of a detector to measure how intense the compound emits light as a result of […]