Category : Chemisty

BCA Protein Concentration Assay

Objective Utilizing Beer’s law, known concentrations of bovine serum albumin (BSA) are reacted with bicinchonic acid (BCA) to create a standard curve of absorbance vs. concentration of protein.  This curve is then utilized to determine the concentration of protein in an unknown solution. Introduction Beer’s law () tells us that the light absorbance (A) of a solution is proportional to the concentration of the protein (c) within the solution, the light path (b) and the extinction coefficient of the protein.  […]

Synthesis of Aspirin

Synthesis of Aspirin By: Jon Torre Purpose: To determine which of four catalysts yields the fastest reaction rate in the acetylation of salicylic acid (1) to form acetylsalicylic acid (2). Reactions: Procedure and Results: Aspirin Synthesis    Tap water was heated on a steam bath in a 250 mL beaker. The temperature of an alcohol thermometer was equilibrated in a beaker of room temperature tap water. Salicylic acid (1.00 g + 0.005 g) and acetic anhydride (2.0 mL + 0.05 mL) were […]

Investigating Polyphosphates with 31P NMR Spectroscopy

Abstract The experiment investigated the use of 31P NMR spectroscopy to determine the structure of polyphosphate anions. The structures of two cyclic (P4O124- and P3O93-) and two linear (P3O105- and P2O74-) were found. Introduction Phosphorus (V) has many polyphosphate oxo-anions which usually existing as either linear or cyclic phosphates. It is known that the linear phosphates are of the form [PnO3n+1](n+2)- and the cyclic phosphates in the form [PnO3n]n- (1). Two of these were synthesised: P3O105- and P4O124- using the […]

Kinetics Lab – Methanolysis of Diethyl Succinate

Abstract: The purpose of this experiment is to determine the rate constant and activation energy in the first step of the methanolysis of diethyl succinate.  The rate constant is determined by analyzing the amount of diethyl succinate present in the reaction mixture at different times in a Gas Chromatogram.  The k1 at 50 degrees Celsius was .0298 while the k1 at 60 degrees Celsius was .0332. Introduction: Gas Chromatography can be used to analyze the kinetics of a reaction that […]

Enzyme Kinetics- Determination of the Kinetic Parameters for Tyrosinase

Enzyme Kinetics- Determination of the Kinetic Parameters for Tyrosinase By:  Trevor Frisby & David Darby Abstract In this Kinetics laboratory experiment the enzyme tyrosinase was investigated in the presence of two types of inhibitors: sodium cyanide and a synthesized inhibitor, dimethoxy azo-stilbene.  Tyrosinase, also known as monophenol monooxygenase utilizes oxygen for the catalysis of two separate reactions that involve the hydroxylation of monophenols to diphenols, and also the oxidative conversion of diphenols to quinones. Through these chemical pathways tyrosinase catalytically […]


IDENTIFICATION OF STEREOCHEMICAL (GEOMETRICAL) ISOMERS OF [Mo(CO)4(L)2] BY IR SPECTROSCOPY. INTRODUCTION Metal carbonyl complexes are of interest in inorganic chemistry as they are good starting materials for many low oxidation state compounds. They are very reactive and lose one or more CO ligands upon heating, photolysis, exposure to other radiation, partial oxidation etc. They react with Lewis bases to form closed shell compounds and can also form clusters. Phosphine complexes (as in this experiment) are also used in many catalytic […]