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Tag : Purifying Proteins

Review of Basic Techniques and Purifying Proteins

Written by Kasia Bender About this lab: Before purifying a protein, a lab has to be able to make solutions of known concentration and deliver small volumes accurately. This review exercise covers both skills: calculating solution concentrations (molarity and percent mass/volume), diluting stocks into a working mix, and checking pipet accuracy by weighing delivered water. The tables below record the student data for each part. Objectives: – To practice calculating various solution components, including molarity and % (m/v). – To […]