Written by Lena ABSTRACT In this experiment, we synthesized luminol and used the product to observe how chemiluminescence works. Our starting material was 5-nitro-2,3-dihydrophthalazine-1,4-dione, which was, after addition of reaction agents, refluxed and vacuum filtered to retrieve luminol. Using two stock solutions, we missed our precipitated luminol with sodium hydroxide, potassium ferricyanide, and hydrogen peroxide, in their respective solutions, in a dark room, to observe the blue light emission. INTRODUCTION Anyone who has watched a CSI show on the television […]
Written by Lauren Objective: To explore the basic principles of genetics such as Medelian genetics using the model organism, Drosophila melanogaster. Introduction: Gregor Mendel, an Austrian monk, observed heritable traits in the common pea plant and discovered several key basic principles in Genetics. The pea plant was perfect organism for Mendel to observe because it reproduces quickly, and in large numbers, and is fairly easy to manipulate. What he discovered was that there are 7 inheritable traits that only have […]
Written by Katie Introduction Methyl Salicylate is a naturally occurring chemical that is obtained from winter green oil that can be used to synthesize Salicylic Acid. Methyl Salicylate is a phenol with a acetyl group in the ortho position. This carbonyl group can undergo typical carbonyl addition-elimination reactions. An addition elimination reaction occurs at the carbonyl carbon where a nucleophile attacks the highly electrophilic carbonyl carbon. The carbonyl carbon is more electrophilic than the other carbons because the double bonded […]
Written by Michael Purpose: The purpose of this experiment was to learn about the use of carbanions and stablilized carbanions in synthesis through performing a synthesis that constructs a carbon-carbon bond. Another purpose of this experiment lies in the purification of an organic base using an acidic aqueous extraction. Experimental: All experimental procedure, observations, and data can be found in Appendix D. Results and Discussion: In the first step, we prepare triphenyl(4-pyridinylmethyl)phosphonium chloride.riphenyl(4-pyridinylmethyl)phosphonium chloride. After the reaction ran to completion, […]
Written by Emily ABSTRACT: Characterizing proteins is useful for determining structural, functional, and other biologically important information. In this experiment, an unknown protein (E) was analyzed, and was found to weigh approximately 50kDa (50,000 g/mol) and have a concentration of 1.56 mg/mL. This data was found using absorbance spectroscopy and SDS-PAGE. These are just two techniques for analyzing proteins out of a plethora of techniques available, and the data gathered from these techniques is useful, but not enough to form […]