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Category : Natural Sciences

Acid-Base extraction of an Analgesic Tablet

The purpose of this experiment was to practice technique and acid-base extraction by separating and purifying the components of a common analgesic tablet. Analysis The purpose of this experiment was to separate and purify the components of an analgesic tablet. Furthermore, the separations allowed for practice of acid-base extractions. This experiment tested laboratory technique, considering that the pill used, Excedrin, contains only milligrams of the drugs to be separated: acetaminophen, caffeine, and aspirin (see Table A for acid/base comparison by […]

Sodium Borohydride Reduction of 2-methylcylohexanone

Purpose The object of this experiment was to reduce the ketone group of a cycloalkane into an alcohol group. This reduction forms cis and trans isomers and the experiment intended to determine which was more favorable. Analysis The object of this experiment was to reduce the ketone group of 2-methylcyclohexanone to form 2-methylcyclohexanol (see figure 1 below). Infrared spectroscopy, an important analytical technique, was introduced to examine the purity of the product. Infrared spectroscopy analyzes a chemical’s transmittance over a […]

Bromination of Cholesterol and Debromination: Purification of Cholesterol

Written by: Nicholas Connor The purpose of this experiment was to understand alkene-halide reactions by purifying commercial cholesterol. Analysis The purpose of this experiment was to remove the impurities present in commercial cholesterol; to yield pure 5-Cholesten-3B-ol. Bromination was the method chosen to remove traces of 3B-cholestanol, 7-cholesten-3b-ol, and 5,7-cholestadien-3B-ol that were assumed to be present in the sample used. The net reaction of bromination follows: To begin removing the impurities, the cholesterol sample (0.97 g) was dissolved in tert-butyl-methyl-ether […]

Lab Report: E. Coli transformation with GFP

E.Coli by Definition: a bacterium commonly found in the intestines of humans and other animals, where it usually causes no harm. Some strains can cause severe food poisoning, especially in old people and children. What organism does this lab attempt to transform? This lab attempts to transform bacterial E. Coli. In the context of this lab, what do we mean when we say a cell is competent? Cellular competence is the ability of a cell to take up genetic material […]

Lab Parts: Evaluating Spring Constants with the Least Squares Method of Data Fitting

Student Researched Lab Experiment to evaluate Spring Constants Introduction In this experiment we evaluated Hooke’s law by measuring the stretch of a spring with incremental masses. A motion detector provided position measurements and the masses were added 100 grams at a time. To calculate the spring constant we utilized the least squares method of data fitting on a Force by distance graph. Sample Calculations Line of Best Fit: f(x) = 0.202x + 16.5 xi-mean height, yi-force of gravity (1) Mean […]

Lab Parts: Ball Toss & Fluid Resistance

Introduction In this experiment, a motion detector was used to collect distance, velocity, and acceleration data for different types of balls being thrown straight up in the air. Like a juggler who tosses a ball straight upward, each ball slows down on it’s path to maximum height, then begins to speed up on it’s way down. The concept is that there is a definite mathematical pattern to the changes in velocity, which will be reinforced by experimentation. Analysis (2) How […]