Purpose: Using H-NMR, in this experiment we sought to study the effect of pH and polarity of the solvent on the Ktrans values of N-Acetyl-L-Proline. The Ktrans values are a means to examine the equilibrium between cis and trans isomers of N-Acetyl-L-Proline. The area under the peak of the H NMR was integrated and divided accordingly to give the Ktransvalues. Ktrans values were used in the calculation of the proportion of cis isomers in the sample. The picture below depicts […]
Give an experimental section for this laboratory experiment. BOTH WEEKS Week 1: Synthesis of a Nickel complex was determined by adding ethylenediamine and ethanol. After obtaining close to 1.000g of nickel(II) sulfate hexahydrate, with your partner as instructed in step one, we quantitatively transferred it to a 250mL beaker with no more than 3mL of water. After the substance had dissolved we added the assigned amount of en to the substance slowly so it could attach to the nickel around […]
Biochemical Activities By: Amanda Goldner Table of Contents Lab A: Media Preparation Lab 1: Inoculations, Smear Preps, and Simple Staining Lab 2: Gram Staining and Using the Spectrophotometer Lab 3: Negative, Acid Fast, Endospore, and Capsule Staining Lab 4: Biochemical Activities of Bacteria I ……………………………………….… 3 Lab 5: Bergey’s Manual (Practical) ………………………………………………… 11 Lab 6: Biochemical Activities of Bacteria II, Unknown Identification, and Rapid Multitests …………………………………………………………………………………………… 13 Lab 7: Isolation of Microorganisms From Soil Lab 8: Eukaryotes I: Fungi Lab […]
Heat Transfer – Pool Boiling in a Saturated Liquid By: Josh Lashbrook Abstract Experiment No. 5, Pool Boiling in a Saturate Liquid, was conducted in order to verify the boiling curve for nitrogen at 1atm, as well as walk through the steps of its original creation. The lab was done using liquid nitrogen and a two submerged copper spheres of different size. Nitrogen was used because of its extremely low boiling point. If water would have been used, time would […]
By: Colin Patrick, Trent McInturff, Michael Billups Abstract This report discusses the methods with which the viscosity of liquid glycerin is determined, and uses the data to validate Stokes’ Law. Two experiments were performed: the viscosity of glycerin was determined using a rotational viscometer, and the data used to validate Stokes’ Law was collected using a falling ball viscometer. Results showed that either method showed significant error and did not validate Stokes’ Law; though the revised Stokes’ Law equation gave […]
By: Ryan DeAngelis, Andrew Mound Introduction The purpose of this experiment is to synthesize an alcohol using hydroboration-oxidation and determine the regiochemistry and stereochemistry of the product via 1H-NMR, 13C-NMR, and Infrared spectroscopy.1 Hydroboration-Oxidation is a two-step process used to convert an alkene to an alcohol. The first step, hydroboration, is the addition of borane (BH3) to the alkene. It proceeds as a concerted mechanism with the electron pair in the pi bond attacking the electrophilic boron, and one of […]